The obtained stain-free model was trained to distinguish between normal, benign, and malignant areas in analyzed specimens using hematoxylin and eosin stained pancreatic tissues isolated from KC (Kras<sup>G12D/+</sup>; Pdx1-Cre) or KPC mice (Kras<sup>G12D/+</sup>; Trp53<sup>R172H/+</sup>; Pdx1-Cre). This evidence concerns the gene PDX1 and keratoconus.