Moreover, it has been documented that in mice, clinical evidence of AKI nephrotoxicity develops 4–6 days after a single dose of CP injection, hence day 11 has been selected as our day of sacrifice, which corresponds to 4 days after CP injection, and this timeline aligns with the protocol that we have applied in our previous studies on CP-induced AKI in mice (Zaaba et al., 2022; Al Za’abi et al., 2021a; Perše and Večerić-Haler, 2018). The gene discussed is CP; the disease is acute kidney injury.