Our RNA-Seq analysis identified Platelet endothelial cell adhesion molecule-1 (PECAM1), type I collagen α2 chain (COL1A2), THBS2 (thrombospondin-2), and type III collagen α1 chain (COL3A1) as potential therapeutic targets in CRC, with each gene being closely associated with fibroblast activity. This evidence concerns the gene COL1A2 and colorectal carcinoma.