According to the analysis of related protein expression based on the mRNA reverse transcription assay, the Hong‐jiou extract affected the mRNA expressions of PPARγ, SREBP‐1c, IRS1, LIPE, and CPT1 to reduce the degree of differentiation and the accumulation of fatty acids as well as increase glucose uptake, thereby having the potential hypoglycemic and anti‐obesity effects. This evidence concerns the gene IRS1 and Obesity.