To assess the relationship between IL-7R and these four cell types in mice with AAAs, we used double immunofluorescence staining to locate the expression of IL-7R in mouse abdominal aortic tissues and labelled these cells with CD31, α-SMA, CD3, and CD68, respectively (Fig. 2A-D). The gene discussed is ACTA1; the disease is achalasia-alacrima syndrome.