Here, using an MRI-based approach, we demonstrated that the age-dependent development of endothelial dysfunction was accelerated by humanised dyslipidaemia in E3L.CETP mice and detected based on Ach response earlier in life of E3L.CETP mice (14 and 22 weeks of age in males and females, respectively) as compared with C57BL/6J mice (40 weeks of age). This evidence concerns the gene CETP and endothelial dysfunction.