To better characterise the phenotype of accelerated, age-dependent endothelial dysfunction development in E3L.CETP mice, biomarkers of the endothelial permeability (Angpt-1, Angpt-2, sFLT-1, sTie-2; Fig. 3A–D), glycocalyx disruption (SDC-1, ESM-1; Fig. 3N–O), endothelial inflammation (sE-sel, sP-sel, sVCAM-1, sICAM-1; Fig. 3J–M), endothelial hemostasis (vWF, PAI-1, t-PA, THBS-1, TAFI; Fig. 3E–I) and other biomarkers (ADN, ADM, ANXA5; Fig. 3P–S) were analysed. Here, SDC1 is linked to endothelial dysfunction.