To characterise the proteomic vascular phenotype in the aorta, associated with accelerated age-dependent endothelial dysfunction in E3L.CETP mice, multidirectional proteomic analysis was performed to characterise changes related to the ageing (8-week-old mice compared to 40-week-old mice within the same strain of mice) and to hyperlipidaemia at 8- and 40-week-old mice (comparison of E3L.CETP mice to age-matched control C57BL/6J mice). Here, CETP is linked to hyperlipidemia.