Finally, we asked which of these genes were repressed in ccRCC tumors versus normal tissue (Supplementary Table S4 - data obtained from the International Cancer Genome Consortium (ICGC)), which resulted in 6 genes (PNPO, ACAT1, DECR1, OAT, SKIDA1 and PSAT1) that fulfilled the following criteria: (1) repressed by pRb in 786-O cells (2) downregulated in VHL-deficient vs VHL-reconstituted 786-O cells (3) downregulated in ccRCC tumor vs normal tissue, and (4) regulated by E2F1 (Fig. 3F). The gene discussed is PNPO; the disease is neoplasm.