Similar to the observed alterations in total SREBP1 protein levels, the nuclear localization of SREBP1, which represents its active form, was also significantly down- or up-regulated when C12ORF49 was silenced or upregulated (Fig. 6G), indicating the activation of SREBP1 by C12ORF49 in HCC cells. Here, SPRING1 is linked to hepatocellular carcinoma.