IFNG and infection: 75,000 C57BL/6J BMDMs were seeded in a 96 well plate and treated for 24 h with 100 U/ml IFNγ and 0.2 μg/ml LPS (InvivoGen 0111:B4), or left untreated as control before infection with RH ∆UPRT, ∆GRA12, ∆GRA12::GRA12-HA in a ratio cell:parasite of 1:5 and a total volume of 100 μl phenol-free media to not interfere with the colorimetric reaction.