And the current reference to TNF generally refers to TNF-α accounting for about 70%-95% of the total TNF bioactivity [46].TNF plays different regulatory roles in different diseases such as coronary heart disease, rheumatoid arthritis, cancer, and ankylosing spondylitis, and especially its anti-tumor effects have received much attention in recent years. Here, TNF is linked to ankylosing spondylitis.