GFAP and Stroke: Given the reliability of GFAP in detecting small thalamic injuries, it is plausible that GFAP could be effective for the detection of other types of injuries such as acute subcortical and cortical strokes.38 However, while our model allows for precise temporal and spatial control of thalamic injury, we acknowledge that the biomarker responses observed in this context may not directly translate to stroke or other types of acute brain insults.