In this study, we hypothetically define the NKG2D+ γδ T-cell subset as the activated subset, the TIGIT+ γδ T-cell subset as exhausted subsets, and the Foxp3+ γδ T-cell subset as the regulatory subset, thus evaluating the expression differences of different functional subsets of γδ T cells in AML patients and healthy controls (HCs) and their correlation with the outcome and prognosis of AML patients. This evidence concerns the gene TIGIT and acute myeloid leukemia.