Inhibition of TORC1 through overexpressing TSC1,2, or overexpressing Proline-rich Akt substrate 40 kDa (PRAS40), or by feeding flies with rapamycin, all induced steatosis in oenocytes (Figure 2E and S2B–C), as observed following Hnf4 knockdown in oenocytes (Figure 1N). This evidence concerns the gene HNF4A and steatosis.