CRISPR-Cas9 AVANA 19Q4 analysis of the MES cell lines also revealed novel genes with a high influence and a strong dependence score, such as ARHGEF5, encoding for the Rho guanine nucleotide exchange factor 5, involved in the EMT process in other types of tumors48, but never studied in GBM. The gene discussed is ARHGEF5; the disease is glioblastoma.