TGFB1 and lung adenocarcinoma: In HEK-293T cells, TGFB1 overexpression increased β-catenin and Snail expression, accompanied by a corresponding downregulation of E-cadherin and Vimentin, indicating that TGFB1 strongly promotes EMT in EGFR-mutant lung adenocarcinoma cells (Fig. 3C, D, G).