In AD like phenotype group the mRNA expression of Secreted Frizzled Related Protein 4 (SFRP4), interleukin 1 alpha (IL-1α), interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), acetyl cholinesterase (AChE), amyloid beta precursor protein (AβPP), beat secretase (β-secretase) significantly (p <  0.05) up-regulated compared to normal control category. Here, TNF is linked to Alzheimer disease.