To further explore whether inhibition of CDK1 pathway or Kinesin family members repress proliferation of these multinucleated cancer cells that persist despite CDK2 inhibition, combined treatment of CYC065 with Ro-3306 (a CDK1 inhibitor) and independently with CYC065 with AZ82 (a KIFC1 inhibitor) were conducted in H1299 and ED1SQ4 cells. The gene discussed is CDK1; the disease is cancer.