Additionally, free fatty acids (FFA) can induce lipid deposition in HepG2 cells, making them more suitable for studying the pathogenesis of MASLD.[16] FFA stimulation significantly increased asprosin protein and mRNA levels in HepG2 cells, and the levels of asprosin in the supernatants of HepG2 cells were also increased by FFA induction (Figure 1k–l). This evidence concerns the gene FBN1 and metabolic dysfunction-associated steatotic liver disease.