To verify the occurrence of pyroptosis after N@VP‐mediated X‐PDT in vivo, we examined the expression of critical pyroptosis‐related proteins in tumor tissues via western blot assay, including domains‐containing protein 3 (NLRP3), cysteine aspartic acid‐specific protease (Caspase1, Casp1), Pro‐casp1, full length gasdermin D (GSDMD‐FL), and cleaved GSDMD (GSDMD‐N) (Figure 7f). This evidence concerns the gene CASP1 and neoplasm.