For those kinases detected in the LC-MS/MS proteomics analysis, none of the kinases with predicted reduced or increased activity showed a difference in abundance between control and T2D iHeps at the protein level, except CHK2, which was higher at the protein level, but predicted to be lower in activity in T2D compared with control iHeps, suggesting that the changes in activity were not likely due to changes in kinase abundance (Supplemental Figure 8). This evidence concerns the gene CHEK2 and type 2 diabetes mellitus.