INS and Insulin resistance: To understand the molecular defects underlying hepatocyte insulin resistance, in the present study, we developed a human iPS cell–derived hepatocyte (iHep) model and used this to define the alterations in basal and insulin-regulated phosphorylation using liquid chromatography with tandem mass spectrometry–based (LC-MS/MS–based) global phosphoproteomics in control individuals and patients with T2D.