TCGA analysis revealed 8,576 differentially expressed genes in both BRCA and normal tissues, and when intersected with the ESCRT gene family, 11 DEGs were identified, including HGS, MVB12A, VPS28, VPS36, VPS37C, VPS37D, VPS 25, CHMP2A, CHMP4B, and CHMP4C, with VPS37D showing the most significant difference in breast cancer (P.adjust <0.05, Figure 6A). Here, HGS is linked to breast carcinoma.