SPP1 and hepatocellular carcinoma: mIF staining validated that SPP1+ macrophages with colocalization of CD68 and SPP1 protein expression, CXCL9+ macrophages with colocalization of CD68 and CXCL9 protein expression and were enriched in HCC tumors (Figures 6E, F, Supplementary Figures 5C, D).