RPS4Y1 and neoplasm: Further analysis utilizing slingshot elucidated the temporal sequence of the four cell subpopulations, revealing that C0 RPS4Y1+ tumor cells are situated at the midpoint of the trajectory and participate in two simulated evolutionary pathways, culminating in C1 LYZ+ tumor cells and C3 MKi67+ tumor cells, respectively (Figures 3E, F).