In TCGA dataset, significant differences in CHRDL1 expression were identified among 22 cancer types, with tumor tissues exhibiting reduced expression compared to normal tissues (p < 0.05), notably in bladder urothelial carcinoma (BLCA), breast invasive carcinoma (BRCA), cervical squamous cell carcinoma (CESC), and colon adenocarcinoma (COAD) (Figure 2B). This evidence concerns the gene CHRDL1 and colon adenocarcinoma.