We report the first known association of HSD11B1 with testosterone-F (burden test P = 1.93 × 10−6 when aggregated across missense variants with MAF <0.01%), with nominal replication in deCODE (P = 0.028); pathogenic variants in this gene are reported to cause hyperandrogenism due to cortisone reductase deficiency49 (Supplementary Fig. 11). Here, HSD11B1 is linked to hyperandrogenism.