To determine the impact of MGAT1 in regulating tumor immune response, we have performed 2D coculture analyses with TNBC breast cancer cells and pre-activated peripheral blood mononuclear cells (PBMCs), followed by 3D coculture analysis using TNBC tumor spheroids and PBMCs, as illustrated in Fig. 2a24. This evidence concerns the gene MGAT1 and breast cancer.