To determine the impact of cytokines on epithelial–mesenchymal transition (EMT), PANC-1, SW620, and MCF-7 cancer cells were treated with TGFβ-1 (4.0 × 10−3 μg/mL), IL-6 (4.1 × 10−5 μg/mL), or HGF (5.97 × 10−4 μg/mL) for 24 h. The gene discussed is IL6; the disease is cancer.