Figure 4 shows how the overlapping hub genes vary in mRNA expression between the two GS cohorts. Six overlapping hub genes (CD8A, CDC20, E2F1, EGF, IL10, TNF, VCAM1) had significantly increased expression in GS 10 patient tumor tissue compared to those with GS 8–9, while one overlapping hub gene (EGF) had significantly lower expression in the tumor tissues of patients with GS 10 compared to those with GS 8–9 (Figure 4). This evidence concerns the gene CD8A and neoplasm.