Third, building upon these findings, our analysis also revealed that several hub genes (CD8A, CDC20, E2F1, IL10, TNF, and VCAM1) were significantly overexpressed in GS 10 tumors compared to patients with GS 8–9, whereas one overlapping hub gene (EGF) was significantly lower in the tumor tissues of patients with GS 10 compared to those with GS 8–9. This evidence concerns the gene E2F1 and neoplasm.