SIRT3 and craniosynostosis 4: However, it is not known how mitochondrial function, redox signaling, and SIRT3 are affected in the heart during CRS-4 development and whether the long-term oral administration of NAC (600 mg/kg/day) can modulate GSH levels and reduce mitochondrial dysfunction, redox signaling, and SIRT3 alterations of the heart in a clinically relevant model of CRS-4 [24,25].