Given that NFκB regulates COX-2 and iNOS, and that endometriosis-associated infertility is often linked to elevated iNOS and NOS enzyme activity, we examined DLBS1442’s ability to modulate iNOS expression. The findings show that DLBS1442 suppressed iNOS mRNA expression in RL95-2 cells, likely due to reduced transcriptional levels of NFκB./Our data suggest that DLBS1442’s anti-inflammatory, analgesic, and anti-angiogenic effects may stem from the downregulation of NFκB activity, leading to reduced expression of iNOS and COX-2. Here, NOS1 is linked to endometriosis.