NPPA and chronic kidney disease: As expected, the expression of COL-1 and MyHC was markedly increased by CKD and retarded in CCN1−/− + CKD mice; consistent with this, similar results were observed between the WT group and the CCN1−/− group; but about the expression of ANP and TGF-β, we found that its expression was increased in the CKD or CCN1−/− + CKD group compared with the WT group or the CCN1−/− group, but there was no significant difference in the CCN1−/− group and WT group or CKD group and CCN1−/− + CKD group.