Additionally, we detected relative hypertrophy and fibrosis markers, and the results showed that the expression levels of TGF-β, COL-1, MyHC, and ANP were significantly decreased in si-CCN1 + CKD serum compared to cells with CKD serum (Figures 5(e) and 5(f)). The gene discussed is TGFB1; the disease is chronic kidney disease.