Immunofluorescence staining further confirmed these observations by showing a substantial increase in myeloperoxidase (MPO)+ myeloid cell infiltration (Figure 4F) and elevated levels of inflammatory cytokines and chemokines, including Tnfa (Figure 4G), Il6 (Figure 4H), Cxcl1 (Figure 4I), Il1β (Supplementary Figure S4A), and Ccl2 (Supplementary Figure S4B), in the pancreas of Lrg1-/- mice following the induction of AP as compared to wild-type counterparts. This evidence concerns the gene TNF and alkaline phosphatase measurement.