Further statistical analysis revealed that elliptical ROI tissues with elevated kio values (> 4 s−1) had significantly higher Ki67 expression than those in ROIs with kio ≤ 4 s−1 (Figure 4G, 30.2% ± 8.2% vs 10.1% ± 7.5%, p < 0.0001, n = (23, 25)), suggesting a reliable association between kio and Ki67 expression throughout glioma progression. Here, MKI67 is linked to central nervous system cancer.