To further confirm the role of intestinal FXR in the obvious effects of DC646 on MASH, Fxr△IE mice were fed with a Gubra-Amylin NASH (GAN) diet for 20 weeks and orally administered with vehicle or 10 mg/kg DC646 daily from the 12th week for 8 weeks (Supplementary Fig. S8a). This evidence concerns the gene NR1H4 and metabolic dysfunction-associated steatohepatitis.