TP53 and B-cell chronic lymphocytic leukemia: Five CLL samples from another institution (Karolinska Hospital) were also analyzed using a third-generation, single-molecule real-time (SMRT) sequencing platform (RS II instrument, PacBio, Menlo Park, California) offering long read lengths able to span the most-frequently mutated region of the TP53 gene [32] These samples, which had been previously tested by NGS and shown to harbor multiple TP53 variants, also showed only trans configuration (Figure 6(f) and supplementary Figure S11A to S11E).