Furthermore, among the genes that had reached maximum genetic and experimental evidence for association with ALS by the ClinGen GCEP as of July 2023 [22]—FUS, OPTN [MIM: 602432], SOD1, SPG11, TARDBP, TBK1 [MIM: 604834], UBQLN2, VAPB, and VCP [MIM: 601023]—all except for SPG11 and VCP showed a >2-fold increase in the proportion of observed supporting pathogenic VUS in pALS than controls (Table S1). This evidence concerns the gene OPTN and amyotrophic lateral sclerosis.