Interestingly, pALS had a greater proportion of VUS that met REVEL's 0.644 cut-off than controls for 15 genes, and no VUSs that exceeded REVEL's 0.644 cut-off were observed in SOD1, TARDBP, TUBA4A, UBQLN2, and VAPB in controls, all of which are definitively associated with ALS [22]. The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.