To further understand the impact of TMEM160 on the biological behavior of GC cells in vitro, we stably overexpressed TMEM160 in SNU-216 cells and knocked down its expression in HGC-27 and BGC-823 cells; siTMEM160 (#1 and #2) and TMEM160 overexpression plasmids exhibited strong knockdown and overexpression efficiencies, respectively (Supplementary Fig. 2A–C). The gene discussed is TMEM160; the disease is gastric cancer.