Subsequent validation in HCT116 cells confirmed a strong interaction between Rap2B and plectin (Fig. 4C), which was further corroborated by the co-immunoprecipitation between endogenous Rap2B and plectin in U2OS osteosarcoma cells and LOVO colorectal cancer cells (Fig. 4D, E). Here, RAP2B is linked to osteosarcoma.