The E2F1 siRNAs were designed, western blotting analysis showed that si-E2F1#1 led to about 5 folds reduction in Huh-7 cells and about 3 folds reduction in Hep3B cells, and si-E2F1#2 induced about 4 folds reduction in Huh-7 cells and about 2.5 folds reduction in Hep3B cells (Fig. 2A), indicating the successful interference of E2F1 in HCC cells. This evidence concerns the gene E2F1 and hepatocellular carcinoma.