In MCD (10–15% fat (lard or soybean oil) and 40–50% carbohydrates)-induced MASLD model within 4–8 weeks, the levels of several fibrosis-related targets, a-Sma, TGF-b1, Col1a1 and Col3a1, were significantly increased. This evidence concerns the gene COL3A1 and metabolic dysfunction-associated steatotic liver disease.