Gebreyesus et al. also demonstrated that female mice with HFD-induced MASLD exhibited the upregulation of PPARγ and the downregulation of anti-obesity genes AMPKα, cytochrome P4502a5 (Cyp2a50), Aldo–keto reductase family 1 member B7 (Akr1b7), Acyl-CoA oxidase 1 (Acox1), but the deletion of pregnane X receptor (Pxr) as a regulator of glycolipid metabolism could suppress MASLD [27]. The gene discussed is NR1I2; the disease is obesity due to melanocortin 4 receptor deficiency.