Our results revealed that PCR-DNA sequencing of fragments of the IL-1α (472 bp), IL-1B (388 bp), IL6 (526 bp), TNF-α (388 bp), IL10 (526 bp), LFA-1 (438 bp), CR2 (381 bp), IL17 (462 bp), IL13 (376 bp), DEFB123 (371 bp), SCART1 (220 bp), ICAM1 (399 bp), SOD1 (387 bp), CAT (308 bp), GPX1 (494 bp), NOS (540 bp), HMOX1 (387 bp), and NQO1 (377 bp) genes revealed nucleotide sequence variations in the form of SNPs between pneumonia-affected and healthy controls Barki ewes. The gene discussed is TNF; the disease is susceptibility to pneumonia measurement.