We next sorted and analyzed Defa6-tdTom tumor cells from the colon tumors of Apc+/Min/ROSA26-tdTomato/Defa6-iCre and Tcf7l2flox/flox/Apc+/Min/ROSA26-tdTomato/Defa6-iCre mice using bulk RNA-seq. Here, APC is linked to neoplasm.