In this study, using the widely accepted Tet-off-induced transgenic CML mice (Scl/tTA-BCR::ABL1) as a model [24], we analyzed significant changes in the metabolic profile at differeny stages following BCR::ABL1 induction and demonstrated that sphingolipid metabolism plays a particularly critical role in the pathogenesis of CML. The gene discussed is BCR; the disease is chronic myelogenous leukemia, BCR-ABL1 positive.