This study primarily investigated the mRNA-level changes to elucidate that differentially expressed genes following SIRT5 interference are predominantly enriched in disease-related pathways, particularly cancer, while potentially regulating lipid deposition in goat intramuscular precursor adipocytes through glycolysis/gluconeogenesis, PI3K-Akt, and MAPK signaling pathways. Here, SIRT5 is linked to cancer.