Although some genes involved in signaling pathways such as the potassium ion channel gene kcnj1a.6 and the gamma-aminobutyric acid A receptor gene gabrp, were down-regulated in spout1-knockout zebrafish, their homologues in humans are either not expressed in the brain or are pseudogenes, suggesting a low likelihood of their contribution to brain development disorders and epilepsy. This evidence concerns the gene SPOUT1 and epilepsy.