In addition, based on IPA analysis, the regulatory effect analysis showed the possible pathways of the upstream regulatory network and downstream molecular function between the genes, in which the PI3K/AKT and TGF-β signaling pathways were correlated with the development of myopia, mainly involving apoptosis, cell viability, and transformation of fibroblasts (Fig. 7C). This evidence concerns the gene AKT1 and myopia.