Inour study, T1DM rats received two daily insulin doses, but the morning insulin dose waswithheld on experiment days to prevent hypoglycemic events that could compromise the study.This insulin withdrawal induced pre-exercise hyperglycemia in T1DM rats, which reachedvalues higher than 300 mg/dL, in contrast to the values of 130-140 mg/dL and 120-125 mg/dLfound in T2DM and normoglycemic rats, respectively. The gene discussed is INS; the disease is type 2 diabetes mellitus.