We found that in all four human cancer types, there were enrichments in the Vps34-activated and Atg14-activated gene signatures in intratumoral Tregs compared to their non-tumor tissue counterparts (Fig 7G), indicating that Vps34 and Atg14 may regulate the function of intratumoral Tregs in human cancer. This evidence concerns the gene PIK3C3 and neoplasm.