FOXO3 and osteosarcoma: Similarly, Lee et al. (2019) showed that ROS generation facilitated tomentosin-induced apoptosis in MG- 63 osteosarcoma cells, with 40 μM tomentosin triggering G2/M arrest, reduced invasion, and upregulated FOXO3 and p27. According to these results, it can be said that tomentosin induces mitochondrial dysfunction, with elevated ROS potentially leading to CRC cell death.