In conclusion, our data suggest that the downregulation of METTL3 inhibits virus replication through the activation of antiviral cytokines in not only PDAC and an immortalized HPDE cell line but also in the A549 lung carcinoma cell line, where METTL3 downregulation leads to heightened cytokine production, particularly IFN-λ1–3. This evidence concerns the gene IFNL1 and lung carcinoma.